| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Maki, Victoria K (15)
|
MAWA |
2:17.79 |
2:13.46 |
-4.33 |
| 2 |
An, Michelle N (15)
|
MAWA |
2:19.59 |
2:21.20 |
1.61 |
| 3 |
Pryshchenko, Katya A (17)
|
MAMSA |
2:24.04 |
2:21.32 |
-2.72 |
| 4 |
Milligan, Hallie A (15)
|
MARKRY |
2:21.67 |
2:23.36 |
1.69 |
| 5 |
Bluestein, Talia R (16)
|
MANSC |
2:30.59 |
2:25.65 |
-4.94 |
| 6 |
Wiley, Hannah E (15)
|
MAUN |
2:35.28 |
2:30.53 |
-4.75 |
| 7 |
Ohsiek, Sarah G (15)
|
MAGRA |
|
2:36.63 |
- |
| 8 |
Girasole-Culhane, Courtnee E (15)
|
MAFSA |
|
2:37.83 |
- |
| 9 |
Nishikawa, Lauren R (16)
|
MAACAC |
2:39.83 |
2:39.85 |
0.02 |
| 10 |
Bossi, Courtney A (17)
|
MACAA |
2:45.05 |
2:43.50 |
-1.55 |
| 11 |
Wilson, Hannah M (17)
|
MAUN |
|
2:53.27 |
- |
| 12 |
Rosa, Kaitlin A (16)
|
MAUN |
2:54.45 |
3:11.08 |
16.63 |